GRB2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
GRB2 polyclonal antibody (Cat # PAB6094) staining (2 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GRB2 polyclonal antibody (Cat # PAB6094) staining (0.3 ug/mL) of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GRB2.
Immunogen
A synthetic peptide corresponding to C-terminus of human GRB2.
Sequence
C-PRNYVTPVNRNV
Host
Goat
Theoretical MW (kDa)
25.2, 20.6
Reactivity
Human, Mouse
Specificity
This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
GRB2 polyclonal antibody (Cat # PAB6094) staining (2 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GRB2 polyclonal antibody (Cat # PAB6094) staining (0.3 ug/mL) of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GRB2
Entrez GeneID
2885Protein Accession#
NP_002077.1;NP_987102.1Gene Name
GRB2
Gene Alias
ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084
Gene Description
growth factor receptor-bound protein 2
Omim ID
108355Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
HT027|OTTHUMP00000166096|OTTHUMP00000166097|OTTHUMP00000166098|abundant SRC homology|epidermal growth factor receptor-binding protein GRB2|growth factor receptor-bound protein 3
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Interactome
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Pathway
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Disease
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Publication Reference
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The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.
Lowenstein EJ, Daly RJ, Batzer AG, Li W, Margolis B, Lammers R, Ullrich A, Skolnik EY, Bar-Sagi D, Schlessinger J.
Cell 1992 Aug; 70(3):431.
Application：IP, WB-Ce, Mouse, HER14 cells.
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The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.
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