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Last updated: 2017/7/23
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GRB2 polyclonal antibody

  • Catalog # : PAB6094
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of GRB2.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human GRB2.
  • Sequence:
  • C-PRNYVTPVNRNV
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 25.2, 20.6
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (1-3 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.1-0.3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • GRB2 polyclonal antibody (Cat # PAB6094) staining (2 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • GRB2 polyclonal antibody (Cat # PAB6094) staining (0.3 ug/mL) of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2885
  • Protein Accession#:
  • NP_002077.1;NP_987102.1
  • Gene Name:
  • GRB2
  • Gene Alias:
  • ASH,EGFRBP-GRB2,Grb3-3,MST084,MSTP084
  • Gene Description:
  • growth factor receptor-bound protein 2
  • Gene Summary:
  • The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • HT027,OTTHUMP00000166096,OTTHUMP00000166097,OTTHUMP00000166098,abundant SRC homology,epidermal growth factor receptor-binding protein GRB2,growth factor receptor-bound protein 3
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