DDAH1 polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of DDAH1.
Immunogen
A synthetic peptide corresponding to human DDAH1.
Sequence
TCCSVLINKKVDS
Host
Goat
Theoretical MW (kDa)
31.1, 20.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
DDAH1 polyclonal antibody (Cat # PAB6093) staining (0.1 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DDAH1
Entrez GeneID
23576Protein Accession#
NP_0.36269.1Gene Name
DDAH1
Gene Alias
DDAH, FLJ21264, FLJ25539
Gene Description
dimethylarginine dimethylaminohydrolase 1
Omim ID
604743Gene Ontology
HyperlinkGene Summary
This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq
Other Designations
NG, NG-dimethylarginine dimethylaminohydrolase|OTTHUMP00000012304
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Interactomes
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Diseases
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Publication Reference
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Purification, cDNA cloning and expression of human NG,NG-dimethylarginine dimethylaminohydrolase.
Kimoto M, Miyatake S, Sasagawa T, Yamashita H, Okita M, Oka T, Ogawa T, Tsuji H.
European Journal of Biochemistry 1998 Dec; 258(2):863.
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Purification, cDNA cloning and expression of human NG,NG-dimethylarginine dimethylaminohydrolase.
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