DDAH2 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DDAH2.
Immunogen
A synthetic peptide corresponding to human DDAH2.
Sequence
SSLCLVLSTRPHS
Host
Goat
Theoretical MW (kDa)
29.6
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
DDAH2 polyclonal antibody (Cat # PAB6092) staining (1 ug/mL) of human heart extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DDAH2
Entrez GeneID
23564Protein Accession#
NP_0.39268.1Gene Name
DDAH2
Gene Alias
DDAH, DDAHII, G6a, NG30
Gene Description
dimethylarginine dimethylaminohydrolase 2
Omim ID
604744Gene Ontology
HyperlinkGene Summary
This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq
Other Designations
NG-dimethylarginine dimethylamino hydrolase homolog|OTTHUMP00000029307|OTTHUMP00000029308|OTTHUMP00000062666|OTTHUMP00000174406|alternative name: NG30, G6a|dimethylarginine dimethylaminohydrolase II
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Interactome
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Disease
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Publication Reference
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Calycosin and formononetin from astragalus root enhance dimethylarginine dimethylaminohydrolase 2 and nitric oxide synthase expressions in Madin Darby Canine Kidney II cells.
Bai F, Makino T, Kono K, Nagatsu A, Ono T, Mizukami H.
Journal of Natural Medicines 2013 Oct; 67(4):782.
Application:WB,ELISA, Dog, MDCK (NBL-2) cells.
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Anti-hypertensive effects of shichimotsukokato in 5/6 nephrectomized Wistar rats mediated by the DDAH-ADMA-NO pathway.
Bai F, Makino T, Ono T, Mizukami H.
Journal of Natural Medicines 2012 Oct; 66(4):583.
Application:WB-Ti, Rat , Rat kidneys.
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Identification of two human dimethylarginine dimethylaminohydrolases with distinct tissue distributions and homology with microbial arginine deiminases.
Leiper JM, Santa Maria J, Chubb A, MacAllister RJ, Charles IG, Whitley GS, Vallance P.
The Biochemical Journal 1999 Oct; 343 Pt 1:209.
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Calycosin and formononetin from astragalus root enhance dimethylarginine dimethylaminohydrolase 2 and nitric oxide synthase expressions in Madin Darby Canine Kidney II cells.
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