CDCP1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
CDCP1 polyclonal antibody (Cat # PAB6088) staining (1 ug/mL) of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing CDCP1 and probed with CDCP1 polyclonal antibody (Cat # PAB6088) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CDCP1.
Immunogen
A synthetic peptide corresponding to C-terminus of human CDCP1.
Sequence
C-PLLNTQEPMEPAE
Host
Goat
Theoretical MW (kDa)
92.3
Reactivity
Human
Specificity
This antibody is expected to recognize isoform 1 (NP_073753.3) only.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CDCP1 polyclonal antibody (Cat # PAB6088) staining (1 ug/mL) of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing CDCP1 and probed with CDCP1 polyclonal antibody (Cat # PAB6088) (mock transfection in first lane), tested by Origene.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDCP1
Entrez GeneID
64866Protein Accession#
NP_073753.3Gene Name
CDCP1
Gene Alias
CD318, SIMA135, TRASK
Gene Description
CUB domain containing protein 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane protein containing three extracellular CUB domains. This protein is found to be overexpressed in colon and lung cancers. Its expression level is correlated with the metastatic ability of carcinoma cells. This protein is located on the cell surface. It has been shown to be tyrosine phosphorylated in a cancer cell line. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
CUB domain-containing protein 1|OTTHUMP00000164120|transmembrane and associated with src kinases
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Interactome
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Publication Reference
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Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen.
Hooper JD, Zijlstra A, Aimes RT, Liang H, Claassen GF, Tarin D, Testa JE, Quigley JP.
Oncogene 2003 Mar; 22(12):1783.
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Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen.
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