NOS1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NOS1.
Immunogen
A synthetic peptide corresponding to human NOS1.
Sequence
C-ESKKDTDEVFSS
Host
Goat
Theoretical MW (kDa)
161
Reactivity
Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NOS1 polyclonal antibody (Cat # PAB6086) staining (0.3 ug/mL) of human muscle extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — NOS1
Entrez GeneID
4842Protein Accession#
NP_000611.1Gene Name
NOS1
Gene Alias
IHPS1, NOS, nNOS
Gene Description
nitric oxide synthase 1 (neuronal)
Gene Ontology
HyperlinkGene Summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. This gene encodes a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in this gene are associated with infantile hypertrophic pyloric stenosis type 1
Other Designations
neuronal nitric oxide synthase
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Interactome
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Pathway
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Disease
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Publication Reference
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Expression of two types of nitric oxide synthase mRNA in human neuroblastoma cell lines.
Fujisawa H, Ogura T, Kurashima Y, Yokoyama T, Yamashita J, Esumi H.
Journal of Neurochemistry 1994 Jul; 63(1):140.
Application:WB-Ce, WB-Ti, Human, Rat, Cerebellum, IMR-32, TWG-I-nu, Nagai, SK-N-DZ, HeLa S-3 cells.
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Expression of two types of nitric oxide synthase mRNA in human neuroblastoma cell lines.
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