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Last updated: 2016/12/4
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CRKL polyclonal antibody

  • Catalog # : PAB6084
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of CRKL.
  • Immunogen:
  • A synthetic peptide corresponding to C terminus of human CRKL.
  • Sequence:
  • C-KIFDPQNPDENE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 33.8
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:16000)
    Western Blot (0.01-0.03 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-3.8 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • CRKL polyclonal antibody (Cat # PAB6084) staining (0.01 ug/mL) of K-562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • CRKL polyclonal antibody (Cat # PAB6084) (3.8 ug/mL) staining of paraffin embedded human thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1399
  • Gene Name:
  • CRKL
  • Gene Alias:
  • -
  • Gene Description:
  • v-crk sarcoma virus CT10 oncogene homolog (avian)-like
  • Gene Summary:
  • This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic
  • Other Designations:
  • v-crk avian sarcoma virus CT10 oncogene homolog-like
  • Interactome
  • Interactome
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