PPP2R5A polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PPP2R5A.
Immunogen
A synthetic peptide corresponding to human PPP2R5A.
Sequence
C-AYNMHSILSNTSAE
Host
Goat
Theoretical MW (kDa)
56.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PPP2R5A polyclonal antibody (Cat # PAB6073) staining (0.5 ug/mL) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPP2R5A
Entrez GeneID
5525Protein Accession#
NP_006234.1Gene Name
PPP2R5A
Gene Alias
B56A, MGC131915, PR61A
Gene Description
protein phosphatase 2, regulatory subunit B', alpha isoform
Omim ID
601643Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily. [provided by RefSeq
Other Designations
OTTHUMP00000034891|PP2A, B subunit, B' alpha isoform|PP2A, B subunit, B56 alpha isoform|PP2A, B subunit, PR61 alpha isoform|PP2A, B subunit, R5 alpha isoform|protein phosphatase 2, regulatory subunit B (B56), alpha isoform|serine/threonine protein phospha
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Interactome
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Pathway
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Publication Reference
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Identification of a new family of protein phosphatase 2A regulatory subunits.
McCright B, Virshup DM.
The Journal of Biological Chemistry 1995 Nov; 270(44):26123.
Application:WB-Tr, Human, HEK 293 cells.
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Identification of a new family of protein phosphatase 2A regulatory subunits.
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