PPP2R5E polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PPP2R5E.
Immunogen
A synthetic peptide corresponding to human PPP2R5E.
Sequence
C-LKRGLRRDGIIPT
Host
Goat
Theoretical MW (kDa)
54.7
Reactivity
Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PPP2R5E polyclonal antibody (Cat # PAB6071) staining (0.5 ug/mL) of mouse brain extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPP2R5E
Entrez GeneID
5529Protein Accession#
NP_006237.1Gene Name
PPP2R5E
Gene Alias
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Gene Description
protein phosphatase 2, regulatory subunit B', epsilon isoform
Omim ID
601647Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. [provided by RefSeq
Other Designations
PP2A, B subunit, B' epsilon|PP2A, B subunit, B56 epsilon|PP2A, B subunit, PR61 epsilon|PP2A, B subunit, R5 epsilon|epsilon isoform of regulatory subunit B56, protein phosphatase 2A|protein phosphatase 2, regulatory subunit B (B56), epsilon isoform|serine/
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of a new family of protein phosphatase 2A regulatory subunits.
McCright B, Virshup DM.
The Journal of Biological Chemistry 1995 Nov; 270(44):26123.
Application:WB-Tr, Human, HEK 293 cells.
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Identification of a new family of protein phosphatase 2A regulatory subunits.
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