PPP2CA/PPP2CB polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PPP2CA/PPP2CB.
Immunogen
A synthetic peptide corresponding to human PPP2CA/PPP2CB.
Sequence
C-EPHVTRRTPDYFL
Host
Goat
Theoretical MW (kDa)
35.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The PPP2CA/PPP2CB polyclonal antibody (Cat # PAB6070) staining (1 ug/mL) of human brain extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPP2CA
Entrez GeneID
5515Protein Accession#
NP_002706.1 (Gene ID : 5515);NP_004147.1 (Gene ID : 5516)Gene Name
PPP2CA
Gene Alias
PP2Ac, PP2CA, RP-C
Gene Description
protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Omim ID
176915Gene Ontology
HyperlinkGene Summary
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq
Other Designations
PP2A-alpha|protein phosphatase 2, catalytic subunit, alpha isoform|replication protein C|serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform|serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
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Gene Info — PPP2CB
Entrez GeneID
5516Protein Accession#
NP_002706.1 (Gene ID : 5515);NP_004147.1 (Gene ID : 5516)Gene Name
PPP2CB
Gene Alias
PP2CB
Gene Description
protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
Omim ID
176916Gene Ontology
HyperlinkGene Summary
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
Other Designations
PP2A-beta|protein phosphatase 2, catalytic subunit, beta isoform|protein phosphatase type 2A catalytic subunit|serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform
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Interactome
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Pathway
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Disease
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Publication Reference
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The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A beta catalytic subunit.
Hemmings BA, Wernet W, Mayer R, Maurer F, Hofsteenge J, Stone SR.
Nucleic Acids Research 1988 Dec; 16(23):11366.
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The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A beta catalytic subunit.
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