ARFGAP3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
ARFGAP3 polyclonal antibody (Cat # PAB6069) staining (2 ug/mL) of mouse brain extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ARFGAP3.
Immunogen
A synthetic peptide corresponding to human ARFGAP3.
Sequence
C-NGVVTSIQDRYGS
Host
Goat
Theoretical MW (kDa)
56.9, 52.0
Reactivity
Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ARFGAP3 polyclonal antibody (Cat # PAB6069) staining (2 ug/mL) of mouse brain extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ARFGAP3
Entrez GeneID
26286Protein Accession#
NP_055385.2Gene Name
ARFGAP3
Gene Alias
ARFGAP1, FLJ45618
Gene Description
ADP-ribosylation factor GTPase activating protein 3
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq
Other Designations
ADP-ribosylation factor GTPase activating protein 1|OTTHUMP00000028526
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Interactome
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Pathway
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Disease
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Publication Reference
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Functional characterization of novel human ARFGAP3.
Liu X, Zhang C, Xing G, Chen Q, He F.
FEBS Letters 2001 Feb; 490(1-2):79.
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Functional characterization of novel human ARFGAP3.
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