NFIL3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
NFIL3 polyclonal antibody (Cat # PAB6068) staining (2 ug/mL) of Daudi lysate (RIPA buffer, 20 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NFIL3.
Immunogen
A synthetic peptide corresponding to human NFIL3.
Sequence
C-RLIATQPISASDSG
Host
Goat
Theoretical MW (kDa)
51.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
NFIL3 polyclonal antibody (Cat # PAB6068) staining (2 ug/mL) of Daudi lysate (RIPA buffer, 20 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NFIL3
Entrez GeneID
4783Protein Accession#
NP_005375.2Gene Name
NFIL3
Gene Alias
E4BP4, IL3BP1, NF-IL3A, NFIL3A
Gene Description
nuclear factor, interleukin 3 regulated
Omim ID
605327Gene Ontology
HyperlinkGene Summary
Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).[supplied by OMIM
Other Designations
OTTHUMP00000021633|interleukin-3 promoter transcriptional activator
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Interactome
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Disease
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Publication Reference
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Molecular cloning and characterization of NF-IL3A, a transcriptional activator of the human interleukin-3 promoter.
Zhang W, Zhang J, Kornuc M, Kwan K, Frank R, Nimer SD.
Molecular and Cellular Biology 1995 Nov; 15(11):6055.
Application：WB-Tr, Human, MLA144, S-LB-1 cells.
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Molecular cloning and characterization of NF-IL3A, a transcriptional activator of the human interleukin-3 promoter.
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