DOCK1 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DOCK1.
Immunogen
A synthetic peptide corresponding to human DOCK1.
Sequence
C-TTRKQTSVDSGIVQ
Host
Goat
Theoretical MW (kDa)
215
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (3~4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
DOCK1 polyclonal antibody (Cat # PAB6058) staining (4 ug/mL) of A-431 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence. -
Gene Info — DOCK1
Entrez GeneID
1793Protein Accession#
NP_001371Gene Name
DOCK1
Gene Alias
DOCK180, ced5
Gene Description
dedicator of cytokinesis 1
Omim ID
601403Gene Ontology
HyperlinkGene Summary
This gene product binds to the SH3 domain of CRK protein. It may regulate cell surface extension and may have a role in the cell surface extension of an engulfing cell around a dying cell during apoptosis. [provided by RefSeq
Other Designations
dedicator of cyto-kinesis 1
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Interactome
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Pathway
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Disease
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Publication Reference
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DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the cell membrane.
Hasegawa H, Kiyokawa E, Tanaka S, Nagashima K, Gotoh N, Shibuya M, Kurata T, Matsuda M.
Molecular and Cellular Biology 1996 Apr; 16(4):1770.
Application:IF, IHC, IP, WB-Ce, WB-Tr, Human, Monkey, Mouse, A-431, COS-1, HeLa, NIH/3T3 cells.
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DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the cell membrane.
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