SH3GLB1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
SH3GLB1 polyclonal antibody (Cat # PAB6051) staining (0.3 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SH3GLB1.
Immunogen
A synthetic peptide corresponding to human SH3GLB1.
Sequence
C-QKGKVPITYLELLN
Host
Goat
Theoretical MW (kDa)
40.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SH3GLB1 polyclonal antibody (Cat # PAB6051) staining (0.3 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SH3GLB1
Entrez GeneID
51100Protein Accession#
NP_057093.1Gene Name
SH3GLB1
Gene Alias
Bif-1, CGI-61, KIAA0491, dJ612B15.2
Gene Description
SH3-domain GRB2-like endophilin B1
Omim ID
609287Gene Ontology
HyperlinkOther Designations
Bax-interacting factor 1|OTTHUMP00000011912|SH3-containing protein SH3GLB1|endophilin B1
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Interactome
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Pathway
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Disease
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Publication Reference
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Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis.
Takahashi Y, Karbowski M, Yamaguchi H, Kazi A, Wu J, Sebti SM, Youle RJ, Wang HG.
Molecular and Cellular Biology 2005 Nov; 25(21):9369.
Application：WB-Ce, WB-Tr, Human, Mouse, HeLa cells, MEFs.
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Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis.
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