SET polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
SET polyclonal antibody (Cat # PAB6048) staining (2 ug/mL) of human kidney extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SET.
Immunogen
A synthetic peptide corresponding to human SET.
Sequence
SAPAAKVSKKELNS-C
Host
Goat
Theoretical MW (kDa)
32.1
Reactivity
Human
Specificity
The peptide sequence used (from AAC50460.1) differs by one residue from the RefSeq entry (NP_003002.1) and the three other Genbank protein sequences referenced in the Locuslink entry. It is not known whether this difference is due to a sequencing error or polymophism but this antibody is expected to recognize both sequences.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:1000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SET polyclonal antibody (Cat # PAB6048) staining (2 ug/mL) of human kidney extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SET
Entrez GeneID
6418Protein Accession#
AAC50460.1Gene Name
SET
Gene Alias
2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA
Gene Description
SET nuclear oncogene
Omim ID
600960Gene Ontology
HyperlinkGene Summary
chromatin remodelling factor|inhibitor of granzyme A-activated DNase|inhibitor-2 of protein phosphatase-2A|protein phosphatase ty
Other Designations
HLA-DR-associated protein II|OTTHUMP00000022286|SET translocation (myeloid leukemia-associated)|Template-Activating Factor-I, chromatin remodelling factor|inhibitor of granzyme A-activated DNase|inhibitor-2 of protein phosphatase-2A|protein phosphatase ty
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Interactome
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Disease
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Publication Reference
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15-deoxy-delta12,14-prostaglandin J2-mediated ERK signaling inhibits gram-negative bacteria-induced RelA phosphorylation and interleukin-6 gene expression in intestinal epithelial cells through modulation of protein phosphatase 2A activity.
Ruiz PA, Kim SC, Sartor RB, Haller D.
The Journal of Biological Chemistry 2004 Jun; 279(34):36103.
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15-deoxy-delta12,14-prostaglandin J2-mediated ERK signaling inhibits gram-negative bacteria-induced RelA phosphorylation and interleukin-6 gene expression in intestinal epithelial cells through modulation of protein phosphatase 2A activity.
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