CAPZB polyclonal antibody
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More Files
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Western Blot (Cell lysate)
CAPZB polyclonal antibody (Cat # PAB6046) staining (1 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CAPZB.
Immunogen
A synthetic peptide corresponding to C-terminus of human CAPZB.
Sequence
NDLVEALKRKQQC
Host
Goat
Theoretical MW (kDa)
30.6
Reactivity
Human, Rat
Specificity
This antibody is expected to be specific to the beta2 isoform of capping protein, found in non-muscle cells and should not recognize the beta1 form.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
CAPZB polyclonal antibody (Cat # PAB6046) staining (1 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CAPZB
Entrez GeneID
832Protein Accession#
NP_004921.1Gene Name
CAPZB
Gene Alias
CAPB, CAPPB, CAPZ, MGC104401, MGC129749, MGC129750
Gene Description
capping protein (actin filament) muscle Z-line, beta
Omim ID
601572Gene Ontology
HyperlinkGene Summary
CAPZB is a member of the F-actin capping protein family. This gene encodes the beta subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments. [provided by RefSeq
Other Designations
Cap Z|F-actin capping protein beta subunit
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Interactome
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Disease
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Publication Reference
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Vertebrate isoforms of actin capping protein beta have distinct functions In vivo.
Hart MC, Cooper JA.
The Journal of Cell Biology 1999 Dec; 147(6):1287.
Application：IF, IHC-P, WB-Ti, Mouse, Mouse hearts.
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Vertebrate isoforms of actin capping protein beta have distinct functions In vivo.
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