WRNIP1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of WRNIP1.
Immunogen
A synthetic peptide corresponding to human WRNIP1.
Sequence
EELRGVDFFKQRRC
Host
Goat
Theoretical MW (kDa)
72.1, 69.5
Specificity
This antibody is expected to recognize both reported isoforms according to NP_569079.1 and NP_064520.2.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
WRNIP1 polyclonal antibody (Cat # PAB6045)(1ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — WRNIP1
Entrez GeneID
56897Protein Accession#
NP_569079.1;NP_064520.2Gene Name
WRNIP1
Gene Alias
FLJ22526, RP11-420G6.2, WHIP, bA420G6.2
Gene Description
Werner helicase interacting protein 1
Omim ID
608196Gene Ontology
HyperlinkGene Summary
Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging. The protein encoded by this gene interacts with the N-terminal portion of Werner protein containing the exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene may influence the aging process. Two transcript variants encoding different isoforms have been isolated for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000015944|OTTHUMP00000015945|Werner helicase interacting protein|putative helicase RUVBL
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Interactome
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Publication Reference
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A novel protein interacts with the Werner's syndrome gene product physically and functionally.
Kawabe Yi, Branzei D, Hayashi T, Suzuki H, Masuko T, Onoda F, Heo SJ, Ikeda H, Shimamoto A, Furuichi Y, Seki M, Enomoto T.
The Journal of Biological Chemistry 2001 Jun; 276(23):20364.
Application:IP, WB-Tr, Human , HEK 293 cells.
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A novel protein interacts with the Werner's syndrome gene product physically and functionally.
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