ITK polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
ITK polyclonal antibody (Cat # PAB6038) staining (2 ug/mL) of Jurkat lysate (RIPA buffer, 1.4E+05 cells per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ITK.
Immunogen
A synthetic peptide corresponding to human ITK.
Sequence
C-RLLRQLAEIAESGL
Host
Goat
Theoretical MW (kDa)
71.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ITK polyclonal antibody (Cat # PAB6038) staining (2 ug/mL) of Jurkat lysate (RIPA buffer, 1.4E+05 cells per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ITK
Entrez GeneID
3702Protein Accession#
NP_005537Gene Name
ITK
Gene Alias
EMT, LYK, MGC126257, MGC126258, PSCTK2
Gene Description
IL2-inducible T-cell kinase
Omim ID
186973Gene Ontology
HyperlinkGene Summary
This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq
Other Designations
homolog of mouse T-cell itk/tsk|tyrosine-protein kinase ITK/TSK|tyrosine-protein kinase LYK
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Interactome
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Pathway
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Disease
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Publication Reference
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A novel human tyrosine kinase gene inducible in T cells by interleukin 2.
Tanaka N, Asao H, Ohtani K, Nakamura M, Sugamura K.
FEBS Letters 1993 Jun; 324(1):1.
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A novel human tyrosine kinase gene inducible in T cells by interleukin 2.
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