PTPN11 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
PTPN11 polyclonal antibody (Cat # PAB6037) staining (2 ug/mL) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing PTPN11 and probed with PTPN11 polyclonal antibody (Cat # PAB6037) (mock transfection in first lane), tested by Origene.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PTPN11 polyclonal antibody (Cat # PAB6037)(3.8 ug/mL) staining of paraffin embedded human cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PTPN11.
Immunogen
A synthetic peptide corresponding to C-terminus of human PTPN11.
Sequence
C-YENVGLMQQQKSFR
Host
Goat
Theoretical MW (kDa)
68
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PTPN11 polyclonal antibody (Cat # PAB6037) staining (2 ug/mL) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing PTPN11 and probed with PTPN11 polyclonal antibody (Cat # PAB6037) (mock transfection in first lane), tested by Origene.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PTPN11 polyclonal antibody (Cat # PAB6037)(3.8 ug/mL) staining of paraffin embedded human cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PTPN11
Entrez GeneID
5781Protein Accession#
NP_002825.3Gene Name
PTPN11
Gene Alias
BPTP3, CFC, MGC14433, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2
Gene Description
protein tyrosine phosphatase, non-receptor type 11
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq
Other Designations
protein tyrosine phosphatase-2|protein-tyrosine phosphatase 2C
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Interactome
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Pathway
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Disease
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Publication Reference
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The SH2 tyrosine phosphatase shp2 is required for mammalian limb development.
Saxton TM, Ciruna BG, Holmyard D, Kulkarni S, Harpal K, Rossant J, Pawson T.
Nature Genetics 2000 Apr; 24(4):420.
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The SH2 tyrosine phosphatase shp2 is required for mammalian limb development.
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