GRAP polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
GRAP polyclonal antibody (Cat # PAB6031) staining (0.3 ug/mL) of human thymus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GRAP.
Immunogen
A synthetic peptide corresponding to human GRAP.
Sequence
C-GFFPRSYVQPVHL
Host
Goat
Theoretical MW (kDa)
25.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GRAP polyclonal antibody (Cat # PAB6031) staining (0.3 ug/mL) of human thymus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GRAP
Entrez GeneID
10750Protein Accession#
NP_006604.1Gene Name
GRAP
Gene Alias
MGC64880
Gene Description
GRB2-related adaptor protein
Omim ID
604330Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the GRB2/Sem5/Drk family. This member functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. [provided by RefSeq
Other Designations
growth factor receptor-bound protein 2-related adaptor protein
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Interactome
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Disease
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Publication Reference
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The immunoglobulin tail tyrosine motif upgrades memory-type BCRs by incorporating a Grb2-Btk signalling module.
Engels N, Konig LM, Schulze W, Radtke D, Vanshylla K, Lutz J, Winkler TH, Nitschke L, Wienands J.
Nature Communications 2014 Nov; 5:5456.
Application：WB, Human, DG75 B cells.
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Grap is a novel SH3-SH2-SH3 adaptor protein that couples tyrosine kinases to the Ras pathway.
Feng GS, Ouyang YB, Hu DP, Shi ZQ, Gentz R, Ni J.
The Journal of Biological Chemistry 1996 May; 271(21):12129.
Application：IP, WB-Ce, Human, K-562, MO7e, MO7eR, TF1 cells.
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The immunoglobulin tail tyrosine motif upgrades memory-type BCRs by incorporating a Grb2-Btk signalling module.
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