ORC4L polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
ORC4L polyclonal antibody (Cat # PAB6028, 0.5 ug/mL) staining of rat heart lysate (35 ug protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ORC4L.
Immunogen
A synthetic peptide corresponding to human ORC4L.
Sequence
C-DVRQWATSSLSWL
Host
Goat
Theoretical MW (kDa)
50.4
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot (Tissue lysate)
ORC4L polyclonal antibody (Cat # PAB6028, 0.5 ug/mL) staining of rat heart lysate (35 ug protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ORC4L
Entrez GeneID
5000Protein Accession#
NP_002543Gene Name
ORC4L
Gene Alias
ORC4, ORC4P
Gene Description
origin recognition complex, subunit 4-like (yeast)
Omim ID
603056Gene Ontology
HyperlinkGene Summary
The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown to form a core complex with ORC2L, -3L, and -5L. Three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq
Other Designations
origin recognition complex subunit 4
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Interactome
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Pathway
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Disease
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Publication Reference
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FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication.
Johmura Y, Osada S, Nishizuka M, Imagawa M.
The Journal of Biological Chemistry 2007 Nov; 283(4):2265.
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Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex.
Radichev I, Kwon SW, Zhao Y, DePamphilis ML, Vassilev A.
The Journal of Biological Chemistry 2006 Jun; 281(32):23264.
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Human origins of DNA replication selected from a library of nascent DNA.
Todorovic V, Giadrossi S, Pelizon C, Mendoza-Maldonado R, Masai H, Giacca M.
Molecular Cell 2005 Aug; 19(4):567.
Application：ChIP, Human, HeLa cells.
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FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication.
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