MSC polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
MSC polyclonal antibody (Cat # PAB6020, 1 ug/mL) staining of human bone marrow lysate (35 ug protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MSC.
Immunogen
A synthetic peptide corresponding to human MSC.
Sequence
C-PLKSDWNSLDGIIR
Host
Goat
Theoretical MW (kDa)
23.6
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MSC polyclonal antibody (Cat # PAB6020, 1 ug/mL) staining of human bone marrow lysate (35 ug protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MSC
Entrez GeneID
9242Protein Accession#
AAC15071.1Gene Name
MSC
Gene Alias
ABF-1, ABF1, MYOR, bHLHa22
Gene Description
musculin (activated B-cell factor-1)
Omim ID
603628Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transcriptional repressor capable of binding an E-box element either as a homodimer or as a heterodimer with E2A in vitro. The encoded protein also forms heterodimers with E2A proteins in vivo. This protein is capable of inhibiting the transactivation capability of E47, an E2A protein, in mammalian cells. This gene is a downstream target of the B-cell receptor signal transduction pathway. [provided by RefSeq
Other Designations
activated B-cell factor 1, homolog of mouse musculin|activated B-cell factor-1|musculin
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Interactome
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Disease
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Publication Reference
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Characterization of ABF-1, a novel basic helix-loop-helix transcription factor expressed in activated B lymphocytes.
Massari ME, Rivera RR, Voland JR, Quong MW, Breit TM, van Dongen JJ, de Smit O, Murre C.
Molecular and Cellular Biology 1998 Jun; 18(6):3130.
Application：WB-Ce, Human, DLD-1, LCL, Namalwa cells.
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Characterization of ABF-1, a novel basic helix-loop-helix transcription factor expressed in activated B lymphocytes.
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