FEM1C polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
FEM1C polyclonal antibody (Cat # PAB6015) staining (0.5 ug/mL) of rat kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Cell lysate)
FEM1C polyclonal antibody (Cat # PAB6015). Western blot analysis of FEM1C expression in HepG2.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FEM1C.
Immunogen
A synthetic peptide corresponding to human FEM1C.
Sequence
C-HIPEKLETFVSLHR
Host
Goat
Theoretical MW (kDa)
68.7
Reactivity
Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
FEM1C polyclonal antibody (Cat # PAB6015) staining (0.5 ug/mL) of rat kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Cell lysate)
FEM1C polyclonal antibody (Cat # PAB6015). Western blot analysis of FEM1C expression in HepG2.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FEM1C
Entrez GeneID
56929Protein Accession#
NP_064562.1Gene Name
FEM1C
Gene Alias
EUROIMAGE686608, EUROIMAGE783647, FEM1A, KIAA1785
Gene Description
fem-1 homolog c (C. elegans)
Omim ID
608767Gene Ontology
HyperlinkOther Designations
feminization 1 homolog a
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Interactome
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Disease
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Publication Reference
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F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis.
Chan SL, Tan KO, Zhang L, Yee KS, Ronca F, Chan MY, Yu VC.
The Journal of Biological Chemistry 1999 Nov; 274(45):32461.
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F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis.
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