MELK polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MELK.
Immunogen
A synthetic peptide corresponding to human MELK.
Sequence
KRLVEDILSSCKV
Host
Goat
Theoretical MW (kDa)
74.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MELK polyclonal antibody (Cat # PAB6013) staining (0.1 ug/mL) of human testes lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MELK
Entrez GeneID
9833Protein Accession#
NP_055606.1Gene Name
MELK
Gene Alias
HPK38, KIAA0175
Gene Description
maternal embryonic leucine zipper kinase
Omim ID
607025Gene Ontology
HyperlinkOther Designations
OTTHUMP00000046113|pEg3 kinase
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Interactome
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Publication Reference
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Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family.
Lin ML, Park JH, Nishidate T, Nakamura Y, Katagiri T.
Breast Cancer Research 2007 Feb; 9(1):R17.
Application:WB-Tr, Human, Human mammary epithelial cells (HMECs), MCF-7, T47D cells.
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Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family.
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