PPP1R15A polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
PPP1R15A polyclonal antibody (Cat # PAB6009). Western blot analysis of PPP1R15A expression in human liver.
Western Blot (Cell lysate)
PPP1R15A polyclonal antibody (Cat # PAB6009). Western blot analysis of PPP1R15A expression in Jurkat.
Western Blot (Cell lysate)
PPP1R15A polyclonal antibody (Cat # PAB6009) staining (0.1 ug/mL) of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PPP1R15A.
Immunogen
A synthetic peptide corresponding to C-terminus of human PPP1R15A.
Sequence
C-AAALDLSGRRG
Host
Goat
Theoretical MW (kDa)
73.4
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PPP1R15A polyclonal antibody (Cat # PAB6009). Western blot analysis of PPP1R15A expression in human liver.Western Blot (Cell lysate)
PPP1R15A polyclonal antibody (Cat # PAB6009). Western blot analysis of PPP1R15A expression in Jurkat.Western Blot (Cell lysate)
PPP1R15A polyclonal antibody (Cat # PAB6009) staining (0.1 ug/mL) of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPP1R15A
Entrez GeneID
23645Protein Accession#
NP_055145.3Gene Name
PPP1R15A
Gene Alias
GADD34
Gene Description
protein phosphatase 1, regulatory (inhibitor) subunit 15A
Omim ID
611048Gene Ontology
HyperlinkGene Summary
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq
Other Designations
growth arrest and DNA-damage-inducible 34|protein phosphatase 1, regulatory subunit 15A
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Interactome
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Disease
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Publication Reference
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Effect of chemotherapy-induced DNA repair on oncolytic herpes simplex viral replication.
Aghi M, Rabkin S, Martuza RL.
Journal of the National Cancer Institute 2006 Jan; 98(1):38.
Application：WB-Tr, Human, U87 MG cells.
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Effect of chemotherapy-induced DNA repair on oncolytic herpes simplex viral replication.
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