RPS6KB1 (phospho S424) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of RPS6KB1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S424 of human RPS6KB1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, untreated or treated with serum (10 %, 10 min), using RPS6KB1 (phospho S424) polyclonal antibody (Cat # PAB5785).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using RPS6KB1 (phospho S424) polyclonal antibody (Cat # PAB5785). -
Gene Info — RPS6KB1
Entrez GeneID
6198Gene Name
RPS6KB1
Gene Alias
PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha
Gene Description
ribosomal protein S6 kinase, 70kDa, polypeptide 1
Omim ID
608938Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq
Other Designations
p70 S6 kinase, alpha 1|p70 S6 kinase, alpha 2|ribosomal protein S6 kinase, 70kD, polypeptide 1|serine/threonine kinase 14 alpha
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Interactome
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Publication Reference
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Paclitaxel induces inactivation of p70 S6 kinase and phosphorylation of Thr421 and Ser424 via multiple signaling pathways in mitosis.
Le XF, Hittelman WN, Liu J, McWatters A, Li C, Mills GB, Bast RC Jr.
Oncogene 2003 Jan; 22(4):484.
Application:WB, Human, BT474, SKBr3 cells.
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Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes.
Krause U, Bertrand L, Hue L.
European Journal of Biochemistry 2002 Aug; 269(15):3751.
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Intracellular signaling of angiotensin II-induced p70 S6 kinase phosphorylation at Ser(411) in vascular smooth muscle cells. Possible requirement of epidermal growth factor receptor, Ras, extracellular signal-regulated kinase, and Akt.
Eguchi S, Iwasaki H, Ueno H, Frank GD, Motley ED, Eguchi K, Marumo F, Hirata Y, Inagami T.
The Journal of Biological Chemistry 1999 Dec; 274(52):36843.
Application:WB-Ce, Rat, Rat vascular smooth muscle cells.
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Paclitaxel induces inactivation of p70 S6 kinase and phosphorylation of Thr421 and Ser424 via multiple signaling pathways in mitosis.
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