VASP polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of the extracts from NIH/3T3 cells untreated or treated with forskolin (40 uM, 30 min) using VASP polyclonal antibody (Cat # PAB5624) .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of VASP.
Immunogen
A synthetic peptide corresponding to residues surrounding S157 of human VASP.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of the extracts from NIH/3T3 cells untreated or treated with forskolin (40 uM, 30 min) using VASP polyclonal antibody (Cat # PAB5624) . -
Gene Info — VASP
Entrez GeneID
7408Gene Name
VASP
Gene Alias
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Gene Description
vasodilator-stimulated phosphoprotein
Omim ID
601703Gene Ontology
HyperlinkGene Summary
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Publication Reference
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Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53.
Millard TH, Bompard G, Heung MY, Dafforn TR, Scott DJ, Machesky LM, Futterer K.
The EMBO Journal 2005 Jan; 24(2):240.
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The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat.
Kuhnel K, Jarchau T, Wolf E, Schlichting I, Walter U, Wittinghofer A, Strelkov SV.
PNAS 2004 Nov; 101(49):17027.
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A proline-rich protein binds to the localization element of Xenopus Vg1 mRNA and to ligands involved in actin polymerization.
Zhao WM, Jiang C, Kroll TT, Huber PW.
The EMBO Journal 2001 May; 20(9):2315.
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Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53.
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