EIF4E polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EIF4E.
Immunogen
A synthetic peptide corresponding to residues surrounding S209 of human EIF4E.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extract from NIH/3T3 cells untreated or treated with 10% serum (15 min) , using EIF4E polyclonal antibody (Cat # PAB5342) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EIF4E polyclonal antibody (Cat # PAB5342) . -
Gene Info — EIF4E
Entrez GeneID
1977Gene Name
EIF4E
Gene Alias
CBP, EIF4E1, EIF4EL1, EIF4F, MGC111573
Gene Description
eukaryotic translation initiation factor 4E
Omim ID
133440Gene Ontology
HyperlinkGene Summary
All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM
Other Designations
eIF-4F 25 kDa subunit|eukaryotic translation initiation factor 4E-like 1|mRNA cap-binding protein
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Interactome
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Pathway
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Disease
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Publication Reference
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tRNASer(CGA) differentially regulates expression of wild-type and codon-modified papillomavirus L1 genes.
Gu W, Li M, Zhao WM, Fang NX, Bu S, Frazer IH, Zhao KN.
Nucleic Acids Research 2004 Aug; 32(15):4448.
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Tethered-function analysis reveals that elF4E can recruit ribosomes independent of its binding to the cap structure.
De Gregorio E, Baron J, Preiss T, Hentze MW.
RNA 2001 Jan; 7(1):106.
Application:WB-Tr, Human, HeLa cells.
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The C-terminal domain of eukaryotic protein synthesis initiation factor (eIF) 4G is sufficient to support cap-independent translation in the absence of eIF4E.
Ohlmann T, Rau M, Pain VM, Morley SJ.
The EMBO Journal 1996 Mar; 15(6):1371.
Application:WB-Ce, Rabbit, Rabbit reticulocytes.
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tRNASer(CGA) differentially regulates expression of wild-type and codon-modified papillomavirus L1 genes.
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