MEF2C polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MEF2C.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding T300 of human MEF2C.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of MEF2C (arrow) using rabbit MEF2C polyclonal antibody (Cat # PAB4943). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MEF2C gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with MEF2C polyclonal antibody (Cat # PAB4943) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MEF2C
Entrez GeneID
4208Protein Accession#
NP_002388;Q06413Gene Name
MEF2C
Gene Alias
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Gene Description
myocyte enhancer factor 2C
Omim ID
600662Gene Ontology
HyperlinkGene Summary
polypeptide C (myocyte enhancer factor 2C)
Other Designations
MADS box transcription enhancer factor 2, polypeptide C (myocyte enhancer factor 2C)
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Interactome
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Pathway
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Disease
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Publication Reference
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Membrane hyperpolarization triggers myogenin and myocyte enhancer factor-2 expression during human myoblast differentiation.
Konig S, Hinard V, Arnaudeau S, Holzer N, Potter G, Bader CR, Bernheim L.
The Journal of Biological Chemistry 2004 Apr; 279(27):28187.
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Mammalian vestigial-like 2, a cofactor of TEF-1 and MEF2 transcription factors that promotes skeletal muscle differentiation.
Maeda T, Chapman DL, Stewart AF.
The Journal of Biological Chemistry 2002 Oct; 277(50):48889.
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TEF-1 and MEF2 transcription factors interact to regulate muscle-specific promoters.
Maeda T, Gupta MP, Stewart AF.
Biochemical and Biophysical Research Communications 2002 Jun; 294(4):791.
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Membrane hyperpolarization triggers myogenin and myocyte enhancer factor-2 expression during human myoblast differentiation.
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