Western blot analysis of GRB2 (arrow) using rabbit GRB2 polyclonal antibody (Cat # PAB4939). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GRB2 polyclonal antibody (Cat # PAB4939) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
HT027,OTTHUMP00000166096,OTTHUMP00000166097,OTTHUMP00000166098,abundant SRC homology,epidermal growth factor receptor-binding protein GRB2,growth factor receptor-bound protein 3