GRB2 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GRB2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding Y209 of human GRB2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of GRB2 (arrow) using rabbit GRB2 polyclonal antibody (Cat # PAB4939). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GRB2 polyclonal antibody (Cat # PAB4939) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GRB2
Entrez GeneID
2885Protein Accession#
NP_002077;P62993Gene Name
GRB2
Gene Alias
ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084
Gene Description
growth factor receptor-bound protein 2
Omim ID
108355Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
HT027|OTTHUMP00000166096|OTTHUMP00000166097|OTTHUMP00000166098|abundant SRC homology|epidermal growth factor receptor-binding protein GRB2|growth factor receptor-bound protein 3
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Interactome
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Pathway
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Disease
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Publication Reference
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An analysis of the phosphoproteome of immune cell lines exposed to the immunomodulatory mycotoxin deoxynivalenol.
da Costa AN, Keen JN, Wild CP, Findlay JB.
Biochimica et Biophysica Acta 2011 Jul; 1814(7):850.
Application:Flow Cyt, Human, RPMI1788 cells.
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Coupling of Grb2 to Gab1 mediates hepatocyte growth factor-induced high intensity ERK signal required for inhibition of HepG2 hepatoma cell proliferation.
Kondo A, Hirayama N, Sugito Y, Shono M, Tanaka T, Kitamura N.
The Journal of Biological Chemistry 2008 Jan; 283(3):1428.
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Multiple-state reactions between the epidermal growth factor receptor and Grb2 as observed by using single-molecule analysis.
Morimatsu M, Takagi H, Ota KG, Iwamoto R, Yanagida T, Sako Y.
PNAS 2007 Nov; 104(46):18013.
Application:IF, Human, A431 cells.
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Sprouty2 binds Grb2 at two different proline-rich regions, and the mechanism of ERK inhibition is independent of this interaction.
Martinez N, Garcia-Dominguez CA, Domingo B, Oliva JL, Zarich N, Sanchez A, Gutierrez-Eisman S, Llopis J, Rojas JM.
Cellular Signalling 2007 Nov; 19(11):2277.
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An analysis of the phosphoproteome of immune cell lines exposed to the immunomodulatory mycotoxin deoxynivalenol.
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