HSD17B10 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of HSD17B10 polyclonal antibody (Cat # PAB4902) in mouse kidney tissue lysate. HSD17B10 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry on formalin-fixed and paraffin-embedded human prostate tissue.
(Top) Coloration with the HSD17B10 polyclonal antibody (Cat # PAB4902) at a 1 : 20 concentration.
(Bottom) Antibody adsorbed to the antigen (peptide), negative control.
Disappearance of cytoplasmic staining indicating the antibody is specific.
Data and protocol courtesy of Marie-Helene Levesque, Centre de Recherche du CHUL, Canada.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HSD17B10.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human HSD17B10.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of HSD17B10 polyclonal antibody (Cat # PAB4902) in mouse kidney tissue lysate. HSD17B10 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry on formalin-fixed and paraffin-embedded human prostate tissue.
(Top) Coloration with the HSD17B10 polyclonal antibody (Cat # PAB4902) at a 1 : 20 concentration.
(Bottom) Antibody adsorbed to the antigen (peptide), negative control.
Disappearance of cytoplasmic staining indicating the antibody is specific.
Data and protocol courtesy of Marie-Helene Levesque, Centre de Recherche du CHUL, Canada.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSD17B10
Entrez GeneID
3028Protein Accession#
NP_004484;Q6IBS9Gene Name
HSD17B10
Gene Alias
17b-HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1
Gene Description
hydroxysteroid (17-beta) dehydrogenase 10
Gene Ontology
HyperlinkGene Summary
This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq
Other Designations
17-beta-hydroxysteroid dehydrogenase type 10|3-hydroxy-2-methylbutyryl-CoA dehydrogenase|AB-binding alcohol dehydrogenase|OTTHUMP00000023348|OTTHUMP00000023349|amyloid-beta binding polypeptide|amyloid-beta peptide binding alcohol dehydrogenase|mental reta
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Interactome
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Pathway
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Publication Reference
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ABAD enhances Abeta-induced cell stress via mitochondrial dysfunction.
Takuma K, Yao J, Huang J, Xu H, Chen X, Luddy J, Trillat AC, Stern DM, Arancio O, Yan SS.
FASEB Journal 2005 Apr; 19(6):597.
Application：IHC-P, WB-Ti, Monkey, Mouse, COS cells, Mouse brains.
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Crystal structure of human ABAD/HSD10 with a bound inhibitor: implications for design of Alzheimer's disease therapeutics.
Kissinger CR, Rejto PA, Pelletier LA, Thomson JA, Showalter RE, Abreo MA, Agree CS, Margosiak S, Meng JJ, Aust RM, Vanderpool D, Li B, Tempczyk-Russell A, Villafranca JE.
Journal of Molecular Biology 2004 Sep; 342(3):943.
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ABAD directly links Abeta to mitochondrial toxicity in Alzheimer's disease.
Lustbader JW, Cirilli M, Lin C, Xu HW, Takuma K, Wang N, Caspersen C, Chen X, Pollak S, Chaney M, Trinchese F, Liu S, Gunn-Moore F, Lue LF, Walker DG, Kuppusamy P, Zewier ZL, Arancio O, Stern D, Yan SS, Wu H.
Science 2004 Apr; 304(5669):448.
Application：IEM, IHC, WB, Human, Human Alzheimer brains.
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ABAD enhances Abeta-induced cell stress via mitochondrial dysfunction.
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