HES1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HES1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human HES1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HES1 polyclonal antibody (Cat # PAB4860) in HL-60 cell lysate (35 ug/lane). HES1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HES1
Entrez GeneID
3280Protein Accession#
NP_005515;Q14469Gene Name
HES1
Gene Alias
FLJ20408, HES-1, HHL, HRY, bHLHb39
Gene Description
hairy and enhancer of split 1, (Drosophila)
Omim ID
139605Gene Ontology
HyperlinkGene Summary
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq
Other Designations
hairy and enhancer of split 1|hairy homolog|transcription factor HES-1
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Interactome
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Pathway
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Disease
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Publication Reference
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Expression of differentiation associated protein Hes1 and Hes5 in cervical squamous carcinoma and its precursors.
Liu J, Ye F, Chen H, Lu W, Zhou C, Xie X.
International Journal of Gynecological Cancer 2007 Nov; 17(6):1293.
Application:IHC-P, Human, Human cervical epithelia, cervical squamous carcinoma.
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Differentiation of human mesenchymal stem cells and articular chondrocytes: analysis of chondrogenic potential and expression pattern of differentiation-related transcription factors.
Karlsson C, Brantsing C, Svensson T, Brisby H, Asp J, Tallheden T, Lindahl A.
Journal of Orthopaedic Research 2007 Feb; 25(2):152.
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Expression of differentiation associated protein Hes1 and Hes5 in cervical squamous carcinoma and its precursors.
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