CDC6 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDC6.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CDC6.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CDC6 polyclonal antibody (Cat # PAB4853) in Ramos cell lysate (35 ug/lane). CDC6 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDC6
Entrez GeneID
990Protein Accession#
NP_001245;Q99741Gene Name
CDC6
Gene Alias
CDC18L, HsCDC18, HsCDC6
Gene Description
cell division cycle 6 homolog (S. cerevisiae)
Omim ID
602627Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq
Other Designations
CDC18 (cell division cycle 18, S.pombe, homolog)-like|CDC6 cell division cycle 6 homolog|CDC6-related protein|cell division cycle 6 protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Cdc6 chromatin affinity is unaffected by serine-54 phosphorylation, S-phase progression, and overexpression of cyclin A.
Alexandrow MG, Hamlin JL.
Molecular and Cellular Biology 2004 Feb; 24(4):1614.
Application:WB-Ce, WB-Tr, Human, Mouse, HeLa, 3T3, and CHO cells.
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Caspase-3-mediated cleavage of Cdc6 induces nuclear localization of p49-truncated Cdc6 and apoptosis.
Yim H, Jin YH, Park BD, Choi HJ, Lee SK.
Molecular Biology of the Cell 2003 Oct; 14(10):4250.
Application:WB-Ce, WB-Tr, Human, HeLa, SK-HEP-1 cells.
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Human replication protein Cdc6 prevents mitosis through a checkpoint mechanism that implicates Chk1.
Clay-Farrace L, Pelizon C, Santamaria D, Pines J, Laskey RA.
The EMBO Journal 2003 Feb; 22(3):704.
Application:Func, Human, HeLa cells.
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Cdc6 chromatin affinity is unaffected by serine-54 phosphorylation, S-phase progression, and overexpression of cyclin A.
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