SOST polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of SOST polyclonal antibody (Cat # PAB4841) in mouse liver tissue lysate (35 ug/lane). SOST (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SOST polyclonal antibody (Cat # PAB4841) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SOST.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SOST.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of SOST polyclonal antibody (Cat # PAB4841) in mouse liver tissue lysate (35 ug/lane). SOST (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SOST polyclonal antibody (Cat # PAB4841) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SOST
Entrez GeneID
50964Protein Accession#
NP_079513;Q9BQB4Gene Name
SOST
Gene Alias
VBCH
Gene Description
sclerosteosis
Gene Ontology
HyperlinkGene Summary
Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease. [provided by RefSeq
Other Designations
sclerostin
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Interactome
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Disease
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Publication Reference
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Bone density ligand, Sclerostin, directly interacts with LRP5 but not LRP5G171V to modulate Wnt activity.
Ellies DL, Viviano B, McCarthy J, Rey JP, Itasaki N, Saunders S, Krumlauf R.
Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mine 2006 Nov; 21(11):1738.
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LRP5 mutations linked to high bone mass diseases cause reduced LRP5 binding and inhibition by SOST.
Semenov MV, He X.
The Journal of Biological Chemistry 2006 Dec; 281(50):38276.
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Human genetics of SOST.
Balemans W, Van Hul W.
Journal of Musculoskeletal & Neuronal Interactions 2006 Oct; 6(4):355.
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Bone density ligand, Sclerostin, directly interacts with LRP5 but not LRP5G171V to modulate Wnt activity.
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