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Last updated: 2017/5/21
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MVP polyclonal antibody

  • Catalog # : PAB4800
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of MVP.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MVP.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:100-500)
    Immunohistochemistry (1:50-100)
    Flow cytometry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The MVP polyclonal antibody (Cat # PAB4800) is used in Western blot to detect MVP in A-375 cell lysate.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of MVP (arrow) using rabbit MVP polyclonal antibody (Cat # PAB4800). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MVP gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human lung carcinomareacted with MVP polyclonal antibody (Cat # PAB4800), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of NCI-H292 cells using MVP polyclonal antibody (Cat # PAB4800)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 9961
  • Gene Name:
  • MVP
  • Gene Alias:
  • LRP,VAULT1
  • Gene Description:
  • major vault protein
  • Gene Summary:
  • This gene encodes the major vault protein which is a lung resistance-related protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. This protein mediates drug resistance, perhaps via a transport process. It is widely distributed in normal tissues, and overexpressed in multidrug-resistant cancer cells. The protein overexpression is a potentially useful marker of clinical drug resistance. This gene produces two transcripts by using two alternative exon 2 sequences; however, the open reading frames are the same in both transcripts. [provided by RefSeq
  • Other Designations:
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