MMP14 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The MMP14 polyclonal antibody (Cat # PAB4782) is used in Western blot to detect MMP14 in A-375 cell lysate.
Western Blot (Transfected lysate)
Western blot analysis of MMP14 (arrow) using rabbit MMP14 polyclonal antibody (Cat # PAB4782). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP14 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MMP14 polyclonal antibody (Cat # PAB4782) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using MMP14 polyclonal antibody (Cat # PAB4782)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MMP14.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human MMP14.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The MMP14 polyclonal antibody (Cat # PAB4782) is used in Western blot to detect MMP14 in A-375 cell lysate.Western Blot (Transfected lysate)
Western blot analysis of MMP14 (arrow) using rabbit MMP14 polyclonal antibody (Cat # PAB4782). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP14 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MMP14 polyclonal antibody (Cat # PAB4782) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using MMP14 polyclonal antibody (Cat # PAB4782)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — MMP14
Entrez GeneID
4323Protein Accession#
MM14_HUMANGene Name
MMP14
Gene Alias
MMP-X1, MT1-MMP, MTMMP1
Gene Description
matrix metallopeptidase 14 (membrane-inserted)
Omim ID
600754Gene Ontology
HyperlinkGene Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq
Other Designations
matrix metalloproteinase 14|matrix metalloproteinase 14 (membrane-inserted)|membrane type 1 metalloprotease|membrane-type matrix metalloproteinase 1|membrane-type-1 matrix metalloproteinase
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Interactome
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Pathway
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Disease
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Publication Reference
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cDNA sequence and mRNA tissue distribution of a novel human matrix metalloproteinase with a potential transmembrane segment.
Will H, Hinzmann B.
European Journal of Biochemistry 1995 Aug; 231(3):602.
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Cloning of a human gene potentially encoding a novel matrix metalloproteinase having a C-terminal transmembrane domain.
Takino T, Sato H, Yamamoto E, Seiki M.
Gene 1995 Apr; 155(2):293.
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Membrane-type matrix metalloproteinase (MT-MMP) gene is expressed in stromal cells of human colon, breast, and head and neck carcinomas.
Okada A, Bellocq JP, Rouyer N, Chenard MP, Rio MC, Chambon P, Basset P.
PNAS 1995 Mar; 92(7):2730.
Application：IHC-P, WB-Ce, WB-Ti, Human, Breast fibroadenomas, Breast carcinoma, Cell lines, Colon carcinoma .
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cDNA sequence and mRNA tissue distribution of a novel human matrix metalloproteinase with a potential transmembrane segment.
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