The MMP14 polyclonal antibody (Cat # PAB4782) is used in Western blot to detect MMP14 in A-375 cell lysate.
Western Blot (Transfected lysate)
Western blot analysis of MMP14 (arrow) using rabbit MMP14 polyclonal antibody (Cat # PAB4782). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP14 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MMP14 polyclonal antibody (Cat # PAB4782) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Flow cytometric analysis of MCF-7 cells using MMP14 polyclonal antibody (Cat # PAB4782)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq