MAGED1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The MAGED1 polyclonal antibody (Cat # PAB4756) is used in Western blot to detect MAGED1 in A-549 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAGED1 polyclonal antibody (Cat # PAB4756) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAGED1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human MAGED1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The MAGED1 polyclonal antibody (Cat # PAB4756) is used in Western blot to detect MAGED1 in A-549 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAGED1 polyclonal antibody (Cat # PAB4756) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAGED1
Entrez GeneID
9500Protein Accession#
NP_008917Gene Name
MAGED1
Gene Alias
DLXIN-1, NRAGE
Gene Description
melanoma antigen family D, 1
Omim ID
300224Gene Ontology
HyperlinkGene Summary
This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000023302|OTTHUMP00000023303|OTTHUMP00000023304|OTTHUMP00000023305|neurotrophin receptor-interacting MAGE homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1.
Sasaki A, Masuda Y, Iwai K, Ikeda K, Watanabe K.
The Journal of Biological Chemistry 2002 Jun; 277(25):22541.
Application：WB-Tr, Human, HEK 293 cells.
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Identification of the translational initiation codon in human MAGED1.
Kubu CJ, Goldhawk DG, Barker PA, Verdi JM.
Genomics 2000 Nov; 70(1):150.
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NRAGE, a novel MAGE protein, interacts with the p75 neurotrophin receptor and facilitates nerve growth factor-dependent apoptosis.
Salehi AH, Roux PP, Kubu CJ, Zeindler C, Bhakar A, Tannis LL, Verdi JM, Barker PA.
Neuron 2000 Aug; 27(2):279.
Application：IF, WB-Ce, WB-Tr, WB-Ti, Human, Rat, PC12nnr5, 293 cells, Embryos, Cortex, Cerebellum, Heart, Lung, Liver, Small intestine.
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A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1.
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