MAGEC1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of K-562 cell lysate (35 ug/lane) with MAGEC1 polyclonal antibody (Cat # PAB4755).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAGEC1 polyclonal antibody (Cat # PAB4755) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAGEC1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MAGEC1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K-562 cell lysate (35 ug/lane) with MAGEC1 polyclonal antibody (Cat # PAB4755).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAGEC1 polyclonal antibody (Cat # PAB4755) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAGEC1
Entrez GeneID
9947Protein Accession#
NP_005453Gene Name
MAGEC1
Gene Alias
CT7, MAGE-C1, MGC39366
Gene Description
melanoma antigen family C, 1
Omim ID
300223Gene Ontology
HyperlinkGene Summary
This gene is a member of the melanoma antigen gene (MAGE) family. The proteins of this family are tumor-specific antigens that can be recognized by autologous cytolytic T lymphocytes. This protein contains a large number of unique short repetitive sequences in front of the MAGE-homologous sequence, and therefore is about 800 aa longer than the other MAGE proteins. [provided by RefSeq
Other Designations
OTTHUMP00000024183
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Interactome
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Publication Reference
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CT7 (MAGE-C1) antigen expression in normal and neoplastic tissues.
Jungbluth AA, Chen YT, Busam KJ, Coplan K, Kolb D, Iversen K, Williamson B, Van Landeghem FK, Stockert E, Old LJ.
International Journal of Cancer 2002 Jun; 99(6):839.
Application：IHC-P, Human, Testis.
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Identification of multiple cancer/testis antigens by allogeneic antibody screening of a melanoma cell line library.
Chen YT, Gure AO, Tsang S, Stockert E, Jager E, Knuth A, Old LJ.
PNAS 1998 Jun; 95(12):6919.
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Identification of a new MAGE gene with tumor-specific expression by representational difference analysis.
Lucas S, De Smet C, Arden KC, Viars CS, Lethe B, Lurquin C, Boon T.
Cancer Research 1998 Feb; 58(4):743.
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CT7 (MAGE-C1) antigen expression in normal and neoplastic tissues.
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