MAGEA3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The MAGEA3 polyclonal antibody (Cat # PAB4745) is used in Western blot to detect MAGEA3 in Jurkat cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast cancer tissue withMAGEA3 polyclonal antibody (Cat # PAB4745). Immunohistochemistry of formalin-fixed, paraffin-embedded tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using MAGEA3 polyclonal antibody (Cat # PAB4745) (right histogram) compared to a negative control cell (left histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAGEA3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 283-314 from C-terminus of human MAGEA3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Flow Cytometry (1:10-1:50)
Immunohistochemistry (1:10-1:50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The MAGEA3 polyclonal antibody (Cat # PAB4745) is used in Western blot to detect MAGEA3 in Jurkat cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast cancer tissue withMAGEA3 polyclonal antibody (Cat # PAB4745). Immunohistochemistry of formalin-fixed, paraffin-embedded tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells using MAGEA3 polyclonal antibody (Cat # PAB4745) (right histogram) compared to a negative control cell (left histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis -
Gene Info — MAGEA3
Entrez GeneID
4102Protein Accession#
P43357Gene Name
MAGEA3
Gene Alias
HIP8, HYPD, MAGE3, MAGEA6, MGC14613
Gene Description
melanoma antigen family A, 3
Omim ID
300174Gene Ontology
HyperlinkGene Summary
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq
Other Designations
MAGE-3 antigen|OTTHUMP00000024220|antigen MZ2-D|melanoma-associated antigen 3
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Interactome
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Publication Reference
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The cancer/testis antigen melanoma-associated antigen-A3/A6 is a novel target of fibroblast growth factor receptor 2-IIIb through histone H3 modifications in thyroid cancer.
Kondo T, Zhu X, Asa SL, Ezzat S.
Clinical Cancer Research 2007 Aug; 13(16):4713.
Application：WB, Human, KTC-1thyroid cancer cells.
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The cancer/testis antigen melanoma-associated antigen-A3/A6 is a novel target of fibroblast growth factor receptor 2-IIIb through histone H3 modifications in thyroid cancer.
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