MAGEA12 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of K-562 cell lysate (35 ug/lane) with MAGEA12 polyclonal antibody (Cat # PAB4743).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAGEA12 polyclonal antibody (Cat # PAB4743) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAGEA12.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human MAGEA12.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K-562 cell lysate (35 ug/lane) with MAGEA12 polyclonal antibody (Cat # PAB4743).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAGEA12 polyclonal antibody (Cat # PAB4743) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAGEA12
Entrez GeneID
4111Protein Accession#
NP_005358Gene Name
MAGEA12
Gene Alias
MAGE12
Gene Description
melanoma antigen family A, 12
Omim ID
300177Gene Ontology
HyperlinkGene Summary
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq
Other Designations
OTTHUMP00000024231
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Interactome
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Publication Reference
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Contrary melanoma-associated antigen-A expression at the tumor front and center: A comparative analysis of stage I and IV head and neck squamous cell carcinoma.
Hartmann S, Brisam M, Rauthe S, Driemel O, Brands RC, Rosenwald A, Kübler AC, Müller‑Richter UDA.
Oncology Letters 2016 Oct; 12(4):2942.
Application：IHC-P, Human, Human oral squamous cell carcinoma.
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Comparative genome sequence analysis of the Bpa/Str region in mouse and Man.
Mallon AM, Platzer M, Bate R, Gloeckner G, Botcherby MR, Nordsiek G, Strivens MA, Kioschis P, Dangel A, Cunningham D, Straw RN, Weston P, Gilbert M, Fernando S, Goodall K, Hunter G, Greystrong JS, Clarke D, Kimberley C, Goerdes M, Blechschmidt K, Rump A, Hinzmann B, Mundy CR, Miller W, Poustka A, Herman GE, Rhodes M, Denny P, Rosenthal A, Brown SD.
Genome Research 2000 Jun; 10(6):758.
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The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28.
Rogner UC, Wilke K, Steck E, Korn B, Poustka A.
Genomics 1995 Oct; 29(3):725.
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Cloning and analysis of MAGE-1-related genes.
Ding M, Beck RJ, Keller CJ, Fenton RG.
Biochemical and Biophysical Research Communications 1994 Jul; 202(1):549.
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Contrary melanoma-associated antigen-A expression at the tumor front and center: A comparative analysis of stage I and IV head and neck squamous cell carcinoma.
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