S1PR1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse lung tissue lysate (35 ug/lane) with S1PR1 polyclonal antibody (Cat # PAB4718).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with S1PR1 polyclonal antibody (Cat # PAB4718) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of S1PR1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human S1PR1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse lung tissue lysate (35 ug/lane) with S1PR1 polyclonal antibody (Cat # PAB4718).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with S1PR1 polyclonal antibody (Cat # PAB4718) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Enzyme-linked Immunoabsorbent Assay
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Gene Info — S1PR1
Entrez GeneID
1901Protein Accession#
NP_001391Gene Name
S1PR1
Gene Alias
CHEDG1, D1S3362, ECGF1, EDG-1, EDG1, FLJ58121, S1P1
Gene Description
sphingosine-1-phosphate receptor 1
Omim ID
601974Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq
Other Designations
G protein-coupled sphingolipid receptor|OTTHUMP00000012525|endothelial differentiation, sphingolipid G-protein-coupled receptor, 1|sphingosine 1-phosphate receptor EDG1
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Interactome
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Pathway
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Disease
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Publication Reference
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Transduction of multiple effects of sphingosine 1-phosphate (S1P) on T cell functions by the S1P1 G protein-coupled receptor.
Dorsam G, Graeler MH, Seroogy C, Kong Y, Voice JK, Goetzl EJ.
The Journal of Immunology 2003 Oct; 171(7):3500.
Application：WB-Tr, Mouse, Rat, Th1, HTC4 cells.
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Inhibition of Ca(2+) signalling by the sphingosine 1-phosphate receptor S1P(1).
zu Heringdorf DM, Vincent ME, Lipinski M, Danneberg K, Stropp U, Wang DA, Tigyi G, Jakobs KH.
Cellular Signalling 2003 Jul; 15(7):677.
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Dual regulation of EDG1/S1P(1) receptor phosphorylation and internalization by protein kinase C and G-protein-coupled receptor kinase 2.
Watterson KR, Johnston E, Chalmers C, Pronin A, Cook SJ, Benovic JL, Palmer TM.
The Journal of Biological Chemistry 2002 Feb; 277(8):5767.
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Transduction of multiple effects of sphingosine 1-phosphate (S1P) on T cell functions by the S1P1 G protein-coupled receptor.
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