park polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of park.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of Drosophila park.
Host
Rabbit
Reactivity
Fruit fly
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
The park polyclonal antibody (Cat # PAB4672) is used in Western blot to detect park in, from left to right, mouse kidney, mouse brain, mouse heart, Ramos, and HeLa tissue lysates .Enzyme-linked Immunoabsorbent Assay
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Gene Info — park
Entrez GeneID
40336Protein Accession#
NP_730600;Q7KTX7Gene Name
park
Gene Alias
CG10523, SD01679, dpk
Gene Description
parkin
Gene Ontology
HyperlinkGene Summary
isoform B|parkin CG10523-PC
Other Designations
D-parkin|dParkin|parkin CG10523-PB, isoform B|parkin CG10523-PC, isoform C
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Publication Reference
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RING finger ubiquitin-protein isopeptide ligase Nrdp1/FLRF regulates parkin stability and activity.
Zhong L, Tan Y, Zhou A, Yu Q, Zhou J.
The Journal of Biological Chemistry 2005 Mar; 280(10):9425.
Application:IF, WB-Tr, Human, C33A cells.
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Parkin counteracts symptoms in a Drosophila model of Parkinson's disease.
Haywood AF, Staveley BE.
BMC Neuroscience 2004 Apr; 5:14.
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Drosophila parkin mutants have decreased mass and cell size and increased sensitivity to oxygen radical stress.
Pesah Y, Pham T, Burgess H, Middlebrooks B, Verstreken P, Zhou Y, Harding M, Bellen H, Mardon G.
Development 2004 May; 131(9):2183.
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RING finger ubiquitin-protein isopeptide ligase Nrdp1/FLRF regulates parkin stability and activity.
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