CERK polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The CERK polyclonal antibody (Cat # PAB4648) is used in Western blot to detect CERK in mouse heart tissue lysate .
Western Blot (Cell lysate)
The CERK polyclonal antibody (Cat # PAB4648) is used in Western blot to detect CERK in A2058 cell lysate .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CERK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CERK.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The CERK polyclonal antibody (Cat # PAB4648) is used in Western blot to detect CERK in mouse heart tissue lysate .Western Blot (Cell lysate)
The CERK polyclonal antibody (Cat # PAB4648) is used in Western blot to detect CERK in A2058 cell lysate .Enzyme-linked Immunoabsorbent Assay
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Gene Info — CERK
Entrez GeneID
64781Protein Accession#
NP_073603Gene Name
CERK
Gene Alias
DKFZp434E0211, FLJ21430, FLJ23239, KIAA1646, LK4, MGC131878, dA59H18.2, dA59H18.3, hCERK
Gene Description
ceramide kinase
Omim ID
610307Gene Ontology
HyperlinkGene Summary
CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM
Other Designations
OTTHUMP00000028554|lipid kinase LK4
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Interactome
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Pathway
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Disease
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Publication Reference
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Ceramide kinase promotes Ca2+ signaling near IgG-opsonized targets and enhances phagolysosomal fusion in COS-1 cells.
Hinkovska-Galcheva V, Clark A, VanWay S, Huang JB, Hiraoka M, Abe A, Borofsky M, Kunkel RG, Shanley T, Shayman JA, Lanni F, Petty HR, Boxer LA.
Journal of Lipid Research 2007 Dec; 49(3):531.
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Ceramide kinase promotes Ca2+ signaling near IgG-opsonized targets and enhances phagolysosomal fusion in COS-1 cells.
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