CKM polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The CKM polyclonal antibody (Cat # PAB4619) is used in Western blot to detect CKM in C6 cell lysate .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal musclereacted with CKM polyclonal antibody (Cat # PAB4619), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry
Flow cytometric analysis of HepG2 cells using CKM polyclonal antibody (Cat # PAB4619)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CKM.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CKM.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The CKM polyclonal antibody (Cat # PAB4619) is used in Western blot to detect CKM in C6 cell lysate .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal musclereacted with CKM polyclonal antibody (Cat # PAB4619), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HepG2 cells using CKM polyclonal antibody (Cat # PAB4619)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — CKM
Entrez GeneID
1158Protein Accession#
P06732Gene Name
CKM
Gene Alias
CKMM, M-CK
Gene Description
creatine kinase, muscle
Omim ID
123310Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq
Other Designations
creatine kinase M chain|creatine kinase-M|muscle creatine kinase
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Interactome
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Pathway
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Disease
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