PRKAG3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of PRKAG3 polyclonal antibody (Cat # PAB4581) in mouse brain tissue lysate . PRKAG3 (arrow) was detected using purified PRKAG3 polyclonal antibody (Cat # PAB4581) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKAG3 polyclonal antibody (Cat # PAB4581) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKAG3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human PRKAG3.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of PRKAG3 polyclonal antibody (Cat # PAB4581) in mouse brain tissue lysate . PRKAG3 (arrow) was detected using purified PRKAG3 polyclonal antibody (Cat # PAB4581) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKAG3 polyclonal antibody (Cat # PAB4581) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKAG3
Entrez GeneID
53632Protein Accession#
NP_059127;Q9UGI9Gene Name
PRKAG3
Gene Alias
AMPKG3
Gene Description
protein kinase, AMP-activated, gamma 3 non-catalytic subunit
Omim ID
604976Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq
Other Designations
5'-AMP-activated protein kinase, gamma-3 subunit|AMP-activated protein kinase, non-catalytic gamma-3 subunit|AMPK gamma-3 chain|OTTHUMP00000164095
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Interactome
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Pathway
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Disease
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Publication Reference
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Hypoxia induces expression and activation of AMPK in rat dental pulp cells.
Fukuyama Y, Ohta K, Okoshi R, Suehara M, Kizaki H, Nakagawa K.
Journal of Dental Research 2007 Sep; 86(9):903.
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Hypoxia induces expression and activation of AMPK in rat dental pulp cells.
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