Western blot analysis of PRKAG3 polyclonal antibody (Cat # PAB4581) in mouse brain tissue lysate . PRKAG3 (arrow) was detected using purified PRKAG3 polyclonal antibody (Cat # PAB4581) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKAG3 polyclonal antibody (Cat # PAB4581) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq
5'-AMP-activated protein kinase, gamma-3 subunit,AMP-activated protein kinase, non-catalytic gamma-3 subunit,AMPK gamma-3 chain,OTTHUMP00000164095