PRKAB2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKAB2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PRKAB2.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The PRKAB2 polyclonal antibody (Cat # PAB4576) is used in Western blot to detect PRKAB2 in mouse brain tissue lysate .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKAB2 polyclonal antibody (Cat # PAB4576) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKAB2
Entrez GeneID
5565Protein Accession#
AAKC_HUMANGene Name
PRKAB2
Gene Alias
MGC61468
Gene Description
protein kinase, AMP-activated, beta 2 non-catalytic subunit
Omim ID
602741Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. [provided by RefSeq
Other Designations
5'-AMP-activated protein kinase, beta-2 subunit|AMP-activated protein kinase beta 2 non-catalytic subunit|AMPK beta 2|AMPK beta-2 chain|OTTHUMP00000015910
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Interactome
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Publication Reference
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AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus.
Minokoshi Y, Alquier T, Furukawa N, Kim YB, Lee A, Xue B, Mu J, Foufelle F, Ferre P, Birnbaum MJ, Stuck BJ, Kahn BB.
Nature 2004 Apr; 428(6982):569.
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Variant screening of PRKAB2, a type 2 diabetes mellitus susceptibility candidate gene on 1q in Pima Indians.
Prochazka M, Farook VS, Ossowski V, Wolford JK, Bogardus C.
Molecular and Cellular Probes 2002 Dec; 16(6):421.
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AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus.
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