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Last updated: 2017/8/20
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PKM2 polyclonal antibody

  • Catalog # : PAB4574
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PKM2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 483-513 at C-terminus of human PKM2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Monkey, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:100-500)
    Immunohistochemistry (1:50-1:100)
    Immunofluorescence (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The PKM2 polyclonal antibody (Cat # PAB4574) is used in Western blot to detect PKM2 in HeLa cell lysate .
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PKM2 polyclonal antibody (Cat # PAB4574) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
  • Immunofluorescence
  • Immunofluorescence
  • A : Negative control of HeLa cells without Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).Alexa-Fluor-546 emits orange fluorescence.
    B : Immunofluorescence analysis of PKM2 polyclonal antibody (Cat # PAB4574) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5315
  • Gene Name:
  • PKM2
  • Gene Alias:
  • CTHBP,MGC3932,OIP3,PK3,PKM,TCB,THBP1
  • Gene Description:
  • pyruvate kinase, muscle
  • Gene Summary:
  • This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq
  • Other Designations:
  • OPA-interacting protein 3,PK, muscle type,pyruvate kinase M2,thyroid hormone-binding protein, cytosolic
  • Interactome
  • Interactome
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