PKLR polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The PKLR polyclonal antibody (Cat # PAB4573) is used in Western blot to detect PKLR in mouse liver tissue lysate .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PKLR polyclonal antibody (Cat # PAB4573) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PKLR.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PKLR.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The PKLR polyclonal antibody (Cat # PAB4573) is used in Western blot to detect PKLR in mouse liver tissue lysate .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PKLR polyclonal antibody (Cat # PAB4573) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PKLR
Entrez GeneID
5313Protein Accession#
NP_000289;P30613Gene Name
PKLR
Gene Alias
PK1, PKL, PKR, PKRL, RPK
Gene Description
pyruvate kinase, liver and RBC
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
R-type/L-type pyruvate kinase|pyruvate kinase 1|pyruvate kinase isozyme R/L|pyruvate kinase type L|pyruvate kinase, liver and RBC type|pyruvate kinase, liver and blood cell|red cell/liver pyruvate kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Structure and function of human erythrocyte pyruvate kinase. Molecular basis of nonspherocytic hemolytic anemia.
Valentini G, Chiarelli LR, Fortin R, Dolzan M, Galizzi A, Abraham DJ, Wang C, Bianchi P, Zanella A, Mattevi A.
The Journal of Biological Chemistry 2002 Jun; 277(26):23807.
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Structure and function of human erythrocyte pyruvate kinase. Molecular basis of nonspherocytic hemolytic anemia.
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