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Last updated: 2016/12/4
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PDK4 polyclonal antibody

  • Catalog # : PAB4566
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PDK4.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to internal region of human PDK4.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:50-100)
    Immunohistochemistry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of PDK4 polyclonal antibody (Cat # PAB4566) in HL-60 cell line lysates (35 ug/lane). PDK4 (arrow) was detected using the purified PDK4 polyclonal antibody (Cat # PAB4566) (1 : 60 dilution).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human skeletal musclereacted with PDK4 polyclonal antibody (Cat # PAB4566), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5166
  • Protein Accession#:
  • NP_002603;Q16654
  • Gene Name:
  • PDK4
  • Gene Alias:
  • FLJ40832
  • Gene Description:
  • pyruvate dehydrogenase kinase, isozyme 4
  • Gene Summary:
  • This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq
  • Other Designations:
  • pyruvate dehydrogenase kinase 4,pyruvate dehydrogenase kinase, isoenzyme 4
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