PDK4 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of PDK4 polyclonal antibody (Cat # PAB4565) in CEM cell line lysates (35 ug/lane). PDK4 (arrow) was detected using the purified PDK4 polyclonal antibody (Cat # PAB4565) (1 : 60 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 polyclonal antibody (Cat # PAB4565) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDK4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human PDK4.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PDK4 polyclonal antibody (Cat # PAB4565) in CEM cell line lysates (35 ug/lane). PDK4 (arrow) was detected using the purified PDK4 polyclonal antibody (Cat # PAB4565) (1 : 60 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 polyclonal antibody (Cat # PAB4565) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDK4
Entrez GeneID
5166Protein Accession#
NP_002603;Q16654Gene Name
PDK4
Gene Alias
FLJ40832
Gene Description
pyruvate dehydrogenase kinase, isozyme 4
Omim ID
602527Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq
Other Designations
pyruvate dehydrogenase kinase 4|pyruvate dehydrogenase kinase, isoenzyme 4
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Interactome
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Disease
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Publication Reference
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Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients.
Rosa G, Di Rocco P, Manco M, Greco AV, Castagneto M, Vidal H, Mingrone G.
Obesity Research 2003 Feb; 11(2):176.
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Downregulation of metabolic gene expression in failing human heart before and after mechanical unloading.
Razeghi P, Young ME, Ying J, Depre C, Uray IP, Kolesar J, Shipley GL, Moravec CS, Davies PJ, Frazier OH, Taegtmeyer H.
Cardiology 2002 Jan; 97(4):203.
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Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme in human.
Rowles J, Scherer SW, Xi T, Majer M, Nickle DC, Rommens JM, Popov KM, Harris RA, Riebow NL, Xia J, Tsui LC, Bogardus C, Prochazka M.
The Journal of Biological Chemistry 1996 Sep; 271(37):22376.
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Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients.
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