PDK1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
The PDK1 polyclonal antibody (Cat # PAB4561) is used in Western blot to detect PDK1 in mouse kidney tissue lysate .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PDK1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The PDK1 polyclonal antibody (Cat # PAB4561) is used in Western blot to detect PDK1 in mouse kidney tissue lysate .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDK1
Entrez GeneID
5163Protein Accession#
NP_002601;Q15118Gene Name
PDK1
Gene Alias
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Gene Description
pyruvate dehydrogenase kinase, isozyme 1
Omim ID
602524Gene Ontology
HyperlinkGene Summary
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq
Other Designations
mitochondrial pyruvate dehydrogenase kinase isoenzyme 1|pyruvate dehydrogenase kinase, isoenzyme 1
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Interactome
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Pathway
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Disease
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Publication Reference
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A phosphoserine/threonine-binding pocket in AGC kinases and PDK1 mediates activation by hydrophobic motif phosphorylation.
Frodin M, Antal TL, Dummler BA, Jensen CJ, Deak M, Gammeltoft S, Biondi RM.
The EMBO Journal 2002 Oct; 21(20):5396.
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Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3.
Sato S, Fujita N, Tsuruo T.
The Journal of Biological Chemistry 2002 Oct; 277(42):39360.
Application：WB-Tr, Monkey, COS-7 cells.
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Sphingosine is a novel activator of 3-phosphoinositide-dependent kinase 1.
King CC, Zenke FT, Dawson PE, Dutil EM, Newton AC, Hemmings BA, Bokoch GM.
The Journal of Biological Chemistry 2000 Jun; 275(24):18108.
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A phosphoserine/threonine-binding pocket in AGC kinases and PDK1 mediates activation by hydrophobic motif phosphorylation.
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